Validation of Developed Analytical Method for Valsartan Floating Tablets by Reverse Phase High Performance Liquid Chromatography

C. Saravanan*1, M. Purushothaman2
1Sun Rise University, Alwar, Rajasthan – 301030, India
2Scient Institute of Pharmacy, Ibrahimpatnam, Ranga Reddy (Dt), Hyderabad, Talangana-501 506, India.

A B S T R A C T
Aim of the present investigation was to validate a new analytical, simple, sensitive, selective and precise High Performance Layer Chromatograpic (HPLC) method for the estimation of Valsartan in tablet dosage form. Valsartan chemically N-(1-oxopentyl)-N-[[2-(1H-tetrazol-5-yl) [1,1’-biphenyl]-4[-yl] methyl] L-Valine used as an Anti-hypertensive agent. The mobile comprised of Acetonitrile: Water (500:500) and set at a flow rate of 1.0ml/minute. Detection was carried out at 273nm using pre-packed Symmetry C18; 250×4.6mm, 5µm particle size column. The retention time of Valsartan was found to be 3.036. The assay was linear over concentration range of 10.00µg/ml to 60µg/ml (R=0.9999). The limit of detection and the limit of quantification were found to be 3.68µg/ml and 11.16µg/ml respectively. The amount of Valsartan was found to be 101.645±1.32 and the accuracy of Valsartan was found to be 98.990% to 101.887%. The statistical analysis of the data showed that the method is reproducible and selective for the estimation of Valsartan in tablet dosage form during routine analysis.
Keywords: Valsartan, RP-HPLC, Validation, Method development