Synthesis and Characterization of Silver Nanoparticles Using Basella Rubra Linn and their In-vitro Cytotoxic Studies

Rajathi.K¹, S.Suja² & N.Kannikaparameswari^3
1Assistant professor, PG and Research Dept. of Biochemistry, Dr. N.G.P Arts and Science College, Kalapatti road, Coimbatore-48.
²Assistant Professor, PG and Research Department of Biochemistry, P.S.G College of Arts and  Science, Coimbatore
³Associate Professor, PG and Research Department of Biochemistry, Dr. N.G.P Arts and Science College, Kalapatti road, Coimbatore-48.

A B S T R A C T
To synthesise the silver nanoparticles using the extracts of Basella rubra Linn and to investigate the antibacterial activity against human pathogens and invitro cytotoxic activity o human breast cancer cell line (MCF-7).In the present study silver nanoparticles were synthesized using the methanolic extract of Basella rubra and were characterized using UV-Visible spectroscopy and SEM analysis. Further these synthesized silver nanoparticles were evaluated for their antibacterial activity against Escherichia coli, Pseudomonas aeroginosa, Staphylococcus aureus and Klebsiella pneumonia by disc diffusion method. The silver nanoparticles were assessed for its cytotoxic activity on human breast cancer cell line (MCF-7).SEM showed the formation of silver nanoparticles with an average size of 20-25nm.Biosynthesised silver nanoparticles showed potent antibactericidal activity against human pathogenic bacteria. The invitro screening of the biosynthesized silver nanoparticles showed potent cytotoxic activity against the human breast cancer cell line.
Keywords: Basella rubra, Silver nanoparticles, human pathogens