Purity of Cardiospermum Helicacabum Linn by Using HPLC Technque in Different Solvents

K. Sudhakar Babu¹, V. Krishna Murthy Naik*¹, J. Latha, V. Prabhakar² and K. Ramanjineyulu
¹Department of Chemistry, Sri Krishnadevaraya University, Anantapuramu, A.P, India
²Department of Bio-technology, Sri Krishnadevaraya University College of Engineering & Technology, S.K. University, Anantapuramu–515003, A.P, India

A B S T R A C T
In this study we report the HPLC Purity of Cardiospermum helicacabum Linn, extract of different solvents, the purity of the biologically active fractions i.e. methanol, chloroform, ethanol and acetone fractions were determined by using in house developed HPLC method and % purity wad reported in the tables 1-4. The corresponding chromatograms The  HPLC chemical investigations has resulted in the identification of the chromatogram, together the crude extract of the Cardiospermum helicacabum Linn flow and stop-flow HPLC analyses rapidly assisted in the identification of the major component , as well as to obtain sufficient quantities for biological testing.
Keywords: Cardiospermum halicacabum Linn. HPLC, Purity, extract.