Optimization and sub-culturing of Verocell culture

Gangadharam Poli¹, Suresh Kumar Chitta¹, Madhusudana Pulaganti³, Prasad Reddy S Hari⁴, C M Anuradha^1,2*
1Department of Bio-Chemistry, Sri Krishnadevaraya University, Anantapuramu, Andhrapradesh, India- 515003.
²Dept of Biotechnology, Sri Krishnadevaraya University, Anantapuramu, Andhrapradesh, India-515003.
³Multidisciplinary Research Unit, SV Medical College, Tirupati, Andhrapradesh, India-517507
⁴Dept of Biotechnology, Koneru Lakshmaiah, Education Foundation, Guntur, Andhrapradesh, India-522502

Abstract
Vero cells are predominant animal cell line widely accepted for viral vaccine manufacturing. Vero cells cannot produce the interferon (antiviral protein) due to inherent genetic defects. This is major advantage of Vero cells used as a substrate for virus propagation in vaccine manufacturing. The regulatory authorities such as World Health Organization (WHO) and Chinese Pharmacopeia are recognized the Vero cells can be used in producing of human biological products. Vero cell lines are stable cell lines, low probability of malignancy, easy to establish the cell bank with different generations because these cells are continuous cell lines. The advances in technology, presently anchorage dependent cells can be produced very large scale, using microcarriers in suspension cultures. This article give an outline of the Vero cells adaptation procedures to serum free media and optimization of adherent cell lines suspension cell cultures using microcarriers technology.
Keywords: Vero cells, Animal cell cultures, Cell biology,Adherent cell lines, Sub culturing, Scale up of cells, bioprocessing, suspension culture, Microcarriers (Cytodex-1)