Investigation of Antioxidant and Antiproliferative activity of Inidigofera trifoliata Linn. against Hep G2 cell line

¹Rajesh.V, G.Mohanraj¹, R.Palani¹ K.Mathivanan¹, S.Sindhu² and P.Jayaraman^1* 
1PG and Research Department of Botany, Government Arts College for Men (Autonomous) Nandanam, Chennai 600 035, Tamil Nadu.
²Armats Biotek Pvt. Ltd., No.14, Mettu street, Maduvankarai, Guindy, Chennai 600032, India
NCADDD, 25 July 2014, Organized by Department of Pharmaceutical Technology, Anna University, BIT Campus, Tiruchirappalli–620024, Tamil Nadu, India

Abstract
The most important phyto-chemical constituents of plants are alkaloids, tannins, flavonoids and phenolic compounds which are capable of curing various human ailments and prevent disorders. Phenolic compounds and flavonoids are widely distributed in plants which have been reported to exert multiple biological effects, including antioxidant, free radical scavenging abilities, anti-carcinogenic activity etc.  Antioxidants are compounds that protect cells against the damaging effects of free radicals such as singlet oxygen, superoxide, hydroxyl radicals and reactive oxygen species. Therefore, the present study is investigated on the effect of antioxidant and anti-proliferative capability of the medicinal plant Indigofera trifoliata Linn. by using leaf extracts with various solvents. Among the solvents like Methanol, Ethyl acetate and Hexane used for extraction, the methanol extract of I. trifoliata observed to be with significant antioxidant capacity by the formation of green phosphomolybdenum complex. The MTT assay was performed to study the anti-proliferative activity of I. trifoliata and found the plant posses potential in scavenging free radicals and in chelating metal ions. In addition, the plant extract proved to be an efficient in causing toxicity to liver cancer cells (Hep G2). Hence, this plant could be considered as an efficient source of therapeutic compounds against free radical induced cancer. However, further studies are required to isolate, purify and analyse the specific bioactive compounds for the antioxidant and anticancer activity.
Keywords: Phyto-chemical analysis, MTT assays, Antioxidant activity, Anti-proliferative activity, Hepatocellular carcinoma (Hep G2), Indigofera trifoliata.