Investigation and comparison of anti-inflammatory effect between methanolic & chloroform extracts with pure molecules (5, 4’ dihydroxy-6.7 methoxy flavone) of roots of Vitex vestita in experimental animals

ABOUT AUTHOR
V. Sreedhar*, Hindustan Abdul Ahad, B. Mohammed Ishaq, G. Naresh Kumar, E. Sateesh Kumar, Shaik Muneer
Department of Pharmaceutics, Balaji College of Pharmacy, Anantapur, AP, India-515001.

Abstract
The objective of present study was evaluating the methanolic & chloroform extract anti-inflammatory activity of vitex vestita. Acute oral toxicity studies in mice of either sex (20-30 g) revealed that the extracts up to 2000 mg/kg have not produced any mortality in experimental animals. Since methanolic extracts have produced lower activity than chloroform extracts which almost equal active with standard drug, the chloroform extract was further subjected to phytochemical evaluation to isolate the pure molecules which are responsible for anti-inflammatory activity. The results were expressed as maximal paw oedema (maximal peak during the 6 h) and as total paw oedema (area under the time-course curve) and presented as mean ± s.e.m., n=5.
Keywords: vitex vestita roots, Carrageenan, inflammation, ibuprofen, methanolic & chloroform extract.