Insulin Secretagogue Effect of Bombax Ceiba on Glucose Induced Insulin Release from Rat Pancreatic Islets

Khan Majid Shabbir¹, I. Sarath Chandiran*², P.D. Chaudhari^3
1Research Scholar, Prist Univesity, Vallam, Thanjavur, Tamilnadu, India
²Professor, Ratnam Institute of Pharmacy, Pidathapolur, SPSR Nellore-524346, Andhra Pradesh, India
³Principal, Modern College of Pharmacy, Nigdi, Pune-44, Maharashtra, India.

A B S T R A C T
In the present study, the effect of methanolic leaf extract of Bombax ceiba (MEBC) was studied on glucose induced insulin secretion in rat pancreas with an evaluated of their mechanism of action. Isolation and preparation of pancreas by collagenase digestion method and Insulin secretion from islets were observed with graduate concentrations of MEBC. There was a concentration-dependent increase in insulin release when islets were incubated with various concentrations of MEBC at 10 and 60 min. For inhibition of insulin from pancreas, the pancreas islets were pre-incubated with diazoxide along with and without 100 mg of MEBC. Incubation of pancreatic islets with glucose and 100mg of MEBC in the presence of diazoxide caused a 22.16% (10 min) and 16.64 % (60 min) inhibition in insulin release. This study clearly indicated that Bombax ceiba reverse the effect of diazoxide. It can be concluded that the insulin secretagogue effect of methanolic extract of leaf of Bombax ceiba regulates the insulin secretion of islets of pancreas because of its antioxidant property.
Keywords: Bombax ceiba, diazoxide, Insulin secretagogue, Glucose induced insulin secretion.