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Maloth Swathi1*, Archana Peddi2
1Department of Pharmaceutics, Sri Shivani College of Pharmacy, Mulugu Road, Warangal, T.S
2Department of Pharmaceutical Chemistry, Netaji Institute of Pharmaceutical Sciences, Choutuppal, Telangana
P-glycoprotein (P-gp) is a transporter protein found in the apical membrane of intestinal mucosal cells, responsible for limiting the bioavailability of orally administered drugs by transporting them back into the intestinal lumen. Compounds that inhibit this drug transport are termed P-gp inhibitors, which can enhance the bioavailability of orally administered drugs. This study aimed to assess the P-gp inhibition activity of selected herbal extracts using Ciprofloxacin as a substrate, employing the in-vitro everted sac method with rat intestine. Ethanolic extracts of pepper, turmeric, ginger, drumstick, cumin, and pomegranate juice were tested for their effect on the intestinal absorption of ciprofloxacin. Drug transport across the intestine was monitored using UV Spectrophotometry at 276nm. Results revealed that 20 mg extracts of pepper, cumin, and pomegranate juice demonstrated a threefold increase, while extracts of ginger and turmeric exhibited a twofold increase in drug absorption, indicating P-gp inhibition activity. However, drumstick extract did not significantly alter drug absorption. Tablets containing the drug and extracts were prepared via wet granulation method, meeting pre- and post-compressionparameters as per pharmacopoeial standards. FT-IR studies revealed no interaction between the drug, extracts, and excipients. Stability studies indicated no physical or chemical changes after two months of storage at specified conditions. These findings suggest that the absorption of P-gp substrates can be enhanced using extracts of pepper, ginger, turmeric, cumin, and pomegranate juice, either individually or in combination.
Keywords: P-glycoprotein, P-gp inhibitors, Ciprofloxacin
