Green synthesis of silver nanoparticles by using Tinospora cordifolia and their evolution of antimicrobial activities and photocatalytic dye degradation studies

Radha Krishna Gandham^{1, 2}, Shivakrishna Pabba², Syama Sundar Bethanabhatla¹*
¹Department of Chemistry, Acharya Nagarjuna University, Nagarjuna Nagar, Guntur-522510, Andhra Pradesh, India.
²Lavin Laboratories, #502, 5^th floor, Windsor plaza, Nallakunta main Road, Beside vegetable market, Hyderabad-500044, Telangana, India.

A B S T R A C T
In this study, rapid and simple approaches for the green synthesis of silver nanoparticles from Tinospora cordifolia aqueous leaf extract (TCLE). The plant extract of TCLE acts as a reducing, stabilizing and capping agent for the synthesis of silver nanoparticles at room temperature. The reaction process was investigated by UV-visible spectrophotometer, FT-IR, HR-SEM. UV-visible spectrophotometer showed absorbance peak in the range of 436–446 nm. To identify the compounds were liable for reduction of silver ions, the functional groups existence in plant extract were FT-IR. The TCLE-Ag NPs showed good antimicrobial activities against both gram positive (Staphylococcus aureus) and gram negative (Escherichia coli, Pseudomonas aerouginosa, Enterobacter aerogenes). They demonstrated highest zone of inhibition (34 nm) towards gram positive (Staphylococcus aureus) microorganism, when compared to other gram negative microorganisms. Moreover, the synthesized silver nanoparticles showed significant photocatytic dye degradation (Methylene blue and Congo red) under UV- light irradiation. Further, the developed TCLE-Ag NPs could be used in the areas of waste water treatment, biomedicine and other biological studies.
Keywords: Tinospora cordifolia, Green synthesis, Silver nanoparticles, Antimicrobial studies, Photocatalytic dye degradation studies.