Flow cytometric evaluation of anti-viral activity of Aegle marmelos against Newcastle disease virus

Amit Gupta*¹, Ramesh B. Jagtap¹ and Sushama R. Chaphalkar^2
1Dept. of Immunology and Virology, Vidya Pratishthan’s School of Biotechnology, Baramati, Pune, Maharashtra India
²Director, Vidya Pratishthan’s School of Biotechnology, Baramati, Pune, Maharashtra, India

A B S T R A C T
In the present study, Aegle marmelos, medicinal plant was screened for antiviral activity against Newcastle Disease Virus (NDV). The results showed that aqueous leaves extract of Aegle marmelos showed inhibitory activity in forward (shape and size) and side scatter (granularity of the cell) at higher doses (10 mg) in chicken lysed whole blood which is confirmed through flow cytometer. In addition, leaves aqueous extract showed rapidly decline in the proliferative response at higher doses as compared to NDV. Results indicated that Aegle marmelos leaves aqueous extract has antiviral property against NDV.
Keywords: Aegle marmelos, flow cytometer, Newcastle disease virus