Extraction of Gallic Acid isolated from Terminalia Chebula, Analysis by Liquid Chromatography−Mass Spectrometry and its Pharmacokinetics in Mice & Rats

Sravan Kumar Reddy Theepireddy*¹, Ravikanth Veluri², Ramakrishna Chinthala¹, L. Vaikunta Rao³, Byresh Pabba², Rathanakar Reddy Kura², Mallikarjuna Rao Duvvada^1
1Department of Environmental Studies, GITAM Institute of Sciences, GITAM University, Visakhapatnam-530045, India
²Hetero Research Foundation, Plot No 80 and 81 A.P.I.E, Balanagar, Hyderabad-500018. India.
³Department of Chemistry, GITAM Institute of Sciences, GITAM University, Visakhapatnam-530045, India.

A B S T R A C T
Gallic acid (GA) is an isolated secondary metabolite from Terminalia chebula, a common medicinal plant regularly used in the traditional preparation of several formulations in Indian system of medicine –Ayurveda. Extraction and processing of plasma samples containing GA from animals is a bit challenge due to its instability. Plasma samples were extracted with and without acidic environment and observed that hydrochloric acid addition has showed better extraction and recovery of GA. Later different formulations have shown very fast absorption and elimination half-life of GA in mice and rats.  
Keywords: Liquid chromatography−electrospray-ionization mass spectrometry, rat plasma, plasma protein binding; Terminalia chebula