CHARACTERIZATION OF THE INTERACTION BETWEEN TWO ANTI-VIRAL DRUGS AND EGG ALBUMIN

ABOUT AUTHOR
S.Bakkialakshmi*,V.Barani
Department of Physics, Annamalai University, Annamalai Nagar, India.
E-mail: bakkialakshmis@rocketmail.com

Abstract
The binding of two anti-viral drugs quercetin and Amantadine to Egg albumin was investigated by fluorescence spectroscopy under simulation of physiological conditions. The quenching mechanism was suggested according to the fluorescence measurement. The Stern-volmer quenching constants were determined. In addition, binding constants were also calculated at room temperature.
Key words: quercetin, Amantadine, Egg albumin (EA), Fluorescence Spectroscopy.
Introduction :
Flavonoids occupy an important position in chemistry and pharmacology. Flavonoids are a group of polyphenolic compounds extensively distributed in the medicinal plants, vegetables, fruit juices and a variety of beverages [tea, coffee, wines and fruit drinks]. Flavonoids particularly quercetin derivatives, have received more attention as dietary constituents during the last few years.  Many studies showed that flavonoids have a wide range of biological activities, such as anticancer, antiviral, antibacterial, antioxidants and anti-inflammatory effects. Flavonoids are best known as radical scavengers. While these valuable effects generally are due to their abilities to accept free radicals, complexation properties with metal ions have also been recognized to contribute to the total biological activity
quercetin is known to a complex with various metal cations to form stable compounds, which have demonstrable antibacterial properties and anti-tumour activity.
Amantadine is an antivirotic drug that has been used to treat influenza and Parkinson disease. Amantadine (1-aminoadamantine) is used against infection with influenza type A virus and to ameliorate symptoms when administered during the early stages of infection, as well as in the management of herpes zoster.  Amantadine is usually given by mouth as the hydrochloride salt.
Albumin is a class of simple, water-soluble proteins that are found in egg white, blood serum, milk, and many other animal and plant fluids and tissues. Albumin has been used as the subject of many investigations because of its important roles in maintaining normal biochemical functions.
Materials and Methods 
Egg Albumin, Amantadine and Quercetin were purchased from Sigma Aldrich Company, Bangalore. Steady-state fluorescence spectra were taken CARRY ECLIPSE VARIAN FLUORESCENCE SPECTROPHOTOMETER.
Results and Discussion 
The steady-state fluorescence spectra of Egg Albumin with different concentrations of Amantadine and Quercetin are shown in figs. 1&2 respectively.  From the figures it can be noted that the fluorescence intensities decrease when the concentration of Amantadine and Quercetin increase. The fluorescence maximum peak occurs at 337 nm.
Conclusion
In this paper, the interaction of the antiviral drugs (quercetin and Amantadine) with EA has been investigated by fluorescence spectroscopic technique under simulated physiological conditions.  This study shows that quercetin and Amantadine binds to Egg albumin and quenches the intrinsic fluorescence of Egg albumin efficiently. binding constants are evaluated and tabulated.
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