Anti-inflammatory activity of flavonoids from medicinal plants against hepatitis B vaccine antigen on human peripheral blood mononuclear cells

Amit Gupta* and Sushama R Chaphalkar
Department of Immunology, Vidya Pratishthan’s School of Biotechnology, Baramati, District Pune, Maharashtra India

A B S T R A C T
The objective of our study is to investigate the anti-inflammatory activity of the variable doses of flavonoids (6.25 – 25 mg) extracted from the leaves of Syzygium cumini, Acacia catechu and Azadirachta indica on human peripheral blood mononuclear cells using specific antigen (i.e. hepatitis vaccine) is to estimate the nitric oxide production from cell culture supernatant and also observed its cytotoxicity. In contrast, these flavonoids also measured its effect on lymphocytes, monocytes and granulocytes count on human whole blood using hepatitis vaccine as antigen which is determined through flow cytometry. The results showed that these flavonoids extracted from these medicinal plants showed maximum inhibition of nitric oxide and cytotoxicity at higher doses. Further, these flavonoids also showed its decline in the level of monocytes count at higher doses as compared to control. The data suggests that flavonoids extracted from these medicinal plants i.e. Syzygium cumini, Acacia catechu and Azadirachta indica showed anti-inflammatory activity.
Keywords: Flavonoids, Syzygium cumini, Acacia catechu, Azadirachta indica