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Pakir Pranaya*
Guru Nanak Institute of Pharmacy, Ibrahimpatnam, Rangareddy, Telangana, India.
A B S T R A C T
The proposed HPLC method was found to be simple, specific, precise, accurate, rapid and economical for simultaneous estimation of Metoprolol and Enalapril Maleate in tablet dosage form. From literature review and solubility analysis initial chromatographic conditions Mobile phase ortho phosphoric acid buffer:Methanol 65:35 were set (Buffer PH 2.45 adjusted with Triethylamine), Kromosil C18 (250×4.6mm, 5µ) Column, Flow rate 1.0ml/min and temperature was ambient, eluent was scanned with PDA detector in system and it showed maximum absorbance at 254 nm. As the methanol content was increased Metoprolol and Enalapril Maleate got eluted with good peak symmetric properties. The retention times for Metprolol and Enalapril Maleate was found to be 2.589 min and 3.711 min respectively. System suitability parameters were studied by injecting the standard five times and results were well under the acceptance criteria. Linearity study was carried out between 50% to150 % levels, R2 value was found to be as 0.999.By using above method assay of marketed formulation was carried out, 100.7% was present. Full length method was not performed; if it is done this method can be used for routine analysis of Metprolol and Enalapril Maleate.
Keywords: HPLC, Enalapril Maleate, Metprolol, Ortho Phosphoric Acid Buffer: Methanol, Kromosil C18
