Analytical Method Development for Sitagliptin and Simvastatin by RP-HPLC Method

D. Yashwanth Kumar^1*, Dr. Rohit Saraswat^2
1Research Scholar, School of Pharmacy, OPJS University, Churu, Rajasthan
²Research Supervisor, School of Pharmacy, OPJS University, Churu, Rajasthan

A B S T R A C T
Analytical chemistry is the qualitative and quantitative analysis of drug substances in biological fluids (mainly plasma and urine) or tissue. It plays a significant role in the evaluation and interpretation of pharmacokinetic data. Method development involves evaluation and optimization of the various stages of sample preparation, chromatographic separation, detection and quantification. An accurate and precise HPLC method was developed for the simultaneous determination of Sitagliptin and Simvastatin. Separation of the drug was achieved on reverse phase ODS C18 column using a mobile phase consisting of Phosphate buffer and Acetonitrile in the ratio of 55:45 v/v. The flow rate was 1ml/min and the detection wavelength was 252nm and peaks were eluted at 2.260 min and 3.683 min.
Keywords: Sitagliptin, Simvastatin, RP-HPLC.