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Abilash Reddy Vancha1, Dr. D. Naresh1, P. Sowjanya1, Dr. Gampa Vijaya Kumar*2
1KGR Institute of Technology and Management, Rampally, Kesara, Rangareddy, Telangana, India.
2Professor and Head, Department of Pharmacy, KGR Institute of Technology and Management, Rampally, Kesara, Rangareddy, Telangana, India
A B S T R A C T
HPLC is at present one of the most sophisticated tool of the analysis. The estimation of elbasvir and grazoprevir was done by RP-HPLC. The Phosphate buffer was pH 3.0 and the mobile phase was optimized with consists of Methanol: Phosphate buffer mixed in the ratio of 70:30 % v/ v. InertsilC18(ODS)column (4.6 x 150mm, 5mm) or equivalent chemically bonded to porous silica particles was used as stationary phase. The detection was carried out using UV detector at 260 nm. The solutions were chromatographed at a constant flow rate of 1.0 ml/min. the linearity range of elbasvir and grazoprevir were found to be from 100-500 mg/ml of elbasvirand 1-5mg/ml of grazoprevir. Linear regression coefficient was not more than 0.999.The values of % RSD are less than 2% indicating accuracy and precision of the method. The percentage recovery varies from 98-102% of elbasvir and grazoprevir. LOD and LOQ were found to be within limit.
Keywords: Metformin, Empagliflozin, RP-HPLC
