Amperometric sensor of Cytosine by heterocyclic polymer fabricated glassy carbon electrode

Brillians Revin^1*, M. Amal Raj^2
1Assistant Professor, Department of Chemistry, Francis Xavier Engineering College, (Post-doctoral Research Scientist, Pusan National University (2012-2014) South Korea), Scad Group of Institutions, Tirunelveli – 627003, Tamilnadu, India.
²Assistant Professor, Department of Chemistry, Loyola College, (Post-doctoral Research Scientist (2014-2015) South Korea) Nungambakkam – 600034, Tamilnadu, India

A B S T R A C T
The electrochemical response for one of the important nucleobases, cytosine (Cyt) is investigated by differential pulse voltammetry (DPV) using an electropolymerized film of 3-amino-5-mercapto-1,2,4-triazole modified glassy carbon (p-AMTa) electrode. Unlike the bare glassy carbon (GC) electrode, p-AMTa electrode shows a well resolved and stable voltammetric signal for Cyt at +1.3 V. In this work, we have achieved a detection of 400 nM of Cyt by amperometry method. Further, the amperometric current response is increased linearly with increasing Cyt concentration in the range of 5.0 × 10^-8 to 1.0 × 10^-4 M and a detection limit is found to be 2.22 × 10^-10 M (S/N = 3). The anti-interference ability of the electrode was also tested with higher concentrations of important interferences.
Keywords: Cytosine, Biosensor, Amperometry, Polymer, Glassy carbon electrode