Histopathological alterations in the kidney of Gambusiaaffinis after exposure to chlorpyrifos

ABOUT AUTHOR
Khan Sabiha*, Sharma Neelam
Department of Zoology
Government College, Ajmer (Raj.) India
E-mail: drsabihakhan786@gmail.com

ABSTRACT
Chlorpyrifos, an organophosphate insecticide, was evaluated for its histopathological effects on fish Gambusiaaffinis, by light microscopy. Chlorpyrifos is a broad-spectrum organophosphate for agriculture. This study aimed to assess the histopathological changes of kidney of fish Gambusiaaffinis. . In the present study Gambusiaaffinis were exposed to 1/10^th of sub-lethal concentration (1/10^th of LC₅₀ value is 0.0284 ppm) of chlorpyrifos, (LC₅₀ is 0.284 ppm) for a period of 15, 30 and 45 days. The treated fish groups were compared with the control group for the histopathological changes in tissues of kidney tissue and marked changes were observed. The renal tissues would be at major toxicological risk since they receive large volumes of blood flow from both the renal portal venous system and the renal arteries. The kidney showed vacuolated epithelial cells, shrinkage of uriniferous tubules, deformed tubules and hypertrophy with pyknotic nuclei of the cells in the renal tubules due to chlorpyrifos toxicity.  These alterations were time and dose dependent.
Key words: Histopathological changes, Gambusiaaffinis, Chlorpyrifos, Kidney