Reverse Phase High Performance Liquid Chromatographic Technique for the Determination of Rabeprazole in Pure and It’s Dosage Form

Dr. Hareesh Dara*, Meesa Rajendar
St John College of Pharmcy, Hasanparthy, Hanamkonda, Telangana.

A B S T R A C T
A rapid and precise reverse phase high performance liquid chromatographic method has been developed for the validated of Rabeprazole, in its pure form as well as in tablet dosage form. Chromatography was carried out on a Symmetry C18 (4.6 x 150mm, 5µm) column using a mixture of Methanol and water (80:20 v/v) as the mobile phase at a flow rate of 0.8ml/min, the detection was carried out at 280nm. The retention time of the Rabeprazole was 2.379 ±0.02min respectively. The method produce linear responses in the concentration range of 20-100mg/ml of Rabeprazole. The method precision for the determination of assay was below 2.0%RSD. The method is useful in the quality control of bulk and pharmaceutical formulations.
Keywords: Rabeprazole, RP-HPLC, validation.