Evaluation of Antidiabetic Activity of Ethanolic Extract of Polyalthia Suberosa Roth in Alloxan Induced Diabetic Rates

G. Harika*¹, Dr. G. Nagarjuna Reddy², Rajani Gunnam³, Dr. J.V. Suresh⁴, G. Sravanthi ^4
1Research Scholar,KLR Pharmacy College, Paloncha, Khammam, Telangana, India
²Department of Pharmaceutical Chemistry, KLR Pharmacy College, Paloncha, Khammam, Telangana, India
³Department of Pharmacy Practice, KLR Pharmacy College, Paloncha, Khammam, Telangana, India
⁴Department of Pharmacology, KLR Pharmacy College, Paloncha, Khammam, Telangana, India

A B S T R A C T
The ethanolic leaf extract of Polyalthia suberosa (EEPS) was tested for its   anti-diabetic activity by normoglycaemic plus glucose fed hyperglycaemic rats (NG-OGTT) method, and sub acute treatment methods in alloxan-induced diabetic rats. In NG-OGTT, Blood glucose levels were determined after oral administration of two concentrations of EEPS (200 and 400 mg/kg) at specific time intervals. In sub acute treatment method, Blood  glucose  levels  were determined  after  oral administration of two concentrations of EEPS (200 and 400 mg/kg) at  0^th, 10^th and 15^th day and biochemical parameters like serum protein, serum creatinine and lipid profile were estimated after 14 days of sub acute study. Glibenclamide (0.4 mg/kg) was taken as a standard reference. The results revealed that EEPS at a dose level of 400 mg/kg exhibit significant (p<0.01) hypoglycaemic effect in NG-OGTT model compared to low dose level of 200 mg/kg (p<0.05). In acute treatment method, both the concentrations of EEPS showed significant (p<0.01) hypoglycaemic effect. In sub acute treated groups administration  of a low dose of 200 mg/kg reduced blood glucose level with less significance (p<0.05) but a high dose of 400 mg/kg reduced blood glucose significantly (p<0.01). In addition, changes in body weight, serum protein, serum creatinine and serum lipid profile levels assessed in the extract treated diabetic rats were compared with standard control, diabetic control and normal animals in sub acute treated groups. Significant results were observed in the estimated parameters further recommended to evaluate EEPS for its long standing anti-diabetic effect and the molecular mechanism for its anti-diabetic activity thereby justifying the use of the plant in the indigenous system of medicine.
Keywords: Polyalthia suberosa, NG-OGTT, Antidiabetic activity, Alloxan.