Method Development and Validation of Nebivolol by RP-HPLC

Naga Jyothi. C*, Gummi Vishwanth, P. Sunitha
Pharmaceutical Analysis and Quality Assurance,   JNTUH, Kukutpally, Hyderabad, India

A B S T R A C T
An isocratic reversed-phase high performance liquid chromatographic method was established for the estimation of Nebivolol in tablet dosage form. The chromatographic conditions were successfully developed for the estimation of Nebivolol by using Develosil C18 column (150×4.6mm, 5µ), flow rate was 1ml/min, mobile phase ratio was (80:20 v/v) Acetonitrile: Phosphate buffer pH 6 (pH was adjusted with diluted phosphoric acid), detection wave length was found to be at 279nm. The Retention time was found to be 2.3mins. The % purity of Nebivolol was found to be 99.56%. Linearity was obtained in the concentration range of 20 µg/ml to 70 µg/ml with correlation coefficient of 0.999. The mean recovery was found to be 100.52%. The Proposed RP-HPLC method was found to be accurate, precise, robust and specific and can be successfully applied for the routine analysis of Nebivolol in tablet dosage form
Keywords: Nebivolol, RP-HPLC, Validation.