Validated RP-HPLC Method for Simultaneous Estimation of Lisinopril and Hydrochlorothiazide in Combined Dosage forms

Author Details
P. Nagaraju*, G. Indira Priyadarshini, SA. Bilal Ahmed and V. Siddhartha
Department of Pharmaceutical Analysis, Hindu College of Pharmacy, Amaravathi Road, Guntur–522 002, Andhra Pradesh, India.

Abstract
A rapid and sensitive reverse phase high performance liquid chromatography (RP-HPLC) method has been developed for simultaneous estimation of Lisinopril and Hydrochlorothiazide. Chromatographic separation was achieved on reverse phase Agilent LC 1100 series HPLC instrument on a Zorbax SB CN column (150 mm x 4.6 mm, 5µ) using the mobile phase consisting of buffer (p^H 6.0) and acetonitrile in the ratio of 70:30 v/v. The mobile phase was pumped at a flow rate of 1.5 ml/min and detection was done by UV detector at 215 nm. The proposed method was found to be simple, fast, accurate, precise and reproducible and could be applied for routine quality control analysis for simultaneous determination of Lisinopril and Hydrochlorothiazide in pharmaceutical dosage forms.
Keywords: Lisinopril, Hydrochlorothiazide, RP-HPLC, validation.