Simultaneous Estimation of Amlodipine and Atenolol by Using Reverse Phase High Performance Liquid Chromatography in Bulk and Marketed Formulation

Devanapelly Shashikantha Rao*
Lecturer, Department of Chemistry, C.K.M. Arts and Science College, Warangal- 506006, Andhra Pradesh, India.

Abstract
A simple precise and accurate reverse phase high performance liquid chromatography (RP-HPLC) method has been developed for the simultaneous estimation of Amlodipine and Atenolol. Chromatographic separation was achieved on Altima C₁₈ column (4.6×150mm, 5µ) using the mobile phase consisting of Methanol: TEA buffer: ACN (50:25:25 v/v). The mobile phase was pumped at a flow rate of 1.0 mL/min and detection was done by UV detector at 225 nm. The retention time for Amlodipine and Atenolol was 2.102 min and 3.577 min. The linearity concentrations for Amlodipine and Atenolol was 5-25 µg/ml and 12.5-62.5 µg/ml respectively with correlation coefficient was found to be 0.999. The proposed method was found to be simple, accurate, precise and cost effective for the simultaneous estimation of Amlodipine and Atenolol in pharmaceutical dosage forms.
Keywords: Amlodipine, Atenolol, RP-HPLC, Mobile phase, TEA Buffer