Simultaneous Estimation Method Development and Validated on Timolol Maleate and Brimonidine Tartrate by Using HPLC

¹N. Kalpana Devi*, ²Sindhu Priya Mattepalli, ³D. S.S.N. Neelima
^1,2 Teegala Krishna Reddy College of Pharmacy, Hyderabad Telangana State
³Vikas Institite of Pharmaceutical Sciences – Rajahmundary

ABSTRACT
A rapid and precise reverse phase high performance liquid chromatographic method has been developed for the validated of Timolol Maleate and Brimonidine Tartrate, in its pure form as well as in tablet dosage form. Chromatography was carried out on an Agilent C18 (4.6×250mm) 5µ column using a mixture of Buffer: Acetonitrile (75:25) as the mobile phase at a flow rate of 0.8ml/min, the detection was carried out at 270nm. The retention time of the Brimonidine Tartrate and Timolol Maleate was 2.0, 3.3±0.02min respectively. The method produce linear responses in the concentration range of 11.25-45µg/ml of Brimonidine Tartrate and 37.5-150µg/ml of Timolol Maleate. The method precision for the determination of assay was below 2.0%RSD. The method is useful in the quality control of bulk and pharmaceutical formulations.
Keywords: Brimonidine Tartrate, Timolol Maleate, RP-HPLC, validation.