Simple Validated UV Method for Tramadol HCL in Bulk and Its Capsules

G. Bhagirathi Bai*, G. Sesha Reddy, M. Kavitha, M. Kanchana, K. Lavanya,
Department of Pharmaceutical Analysis, Balaji College of Pharmacy, Anantapur–515001, AP, India

A B S T R A C T
Tramadol (TMD), is a widely prescribed drug for moderate to severe, acute and chronic pain. The present research work discusses the development and validation of a UV spectrophotometric method for tramadol. Simple, accurate, precise and cost efficient spectrophotometric method has been developed for the estimation of TMD in bulk and its capsules dosage form. The optimum conditions for the analysis of the drug were established. The maximum wavelength (λ max) was found to be 271 nm in water. The mean percentage recovery of TMD was found to be in range 99.29-100.88 %. Beers law was obeyed in the concentration range of 10-200 μg/ml. Calibration curves shows a linear relationship between the absorbance and concentration. The line equation y = 0.0043x + 0.0758 with R² = 0.9997 was obtained. Validation was performed as ICH guidelines for Linearity, accuracy, precision, LOD and LOQ. The proposed method may be suitable for the analysis of TMD in bulk and capsules formulation for routine quality control purposes.
Keywords: Tramadol, chronic pain, UV spectrophotometric, beers law, capsules