RP-HPLC Method Development and Validation of Pimavanserin

J. Praveen Kumar¹, G. Kalyani*, N.Sujani², M.Venkateswarlu³, Dr. S. Veera Sekhar^4
1Associate Professor, Krishna Teja Pharmacy College, Tirupati, A.P., India
^*Srinivasa Institute of Pharmaceutical Sciences, Proddatur, A.P., India.
²Assistant Professor, Srinivasa Institute of Pharmaceutical Sciences, Proddatur, A.P., India.
³Assistant Professor, Krishna Teja Pharmacy College, Tirupati, A.P., India.
⁴Assistant Professor, Srinivasa Institute of Pharmaceutical Sciences, Proddatur, A.P., India.

A  B  S  T  R A C T
New method was established for method development and validation of Pimavanserin by RP-HPLC method. The chromatographic conditions were successfully developed for the separation of Pimavanserin by using Inertsil ODS C18 column (4.6×150mm) 5µ. The wavelength for detection was selected by scanning standard Pimavanserin over a wide range of wavelength 200nm to 400nm. UV spectra at 290nm was selected as the detection wavelength in different mobile phase were tried but satisfactory separation, well resolved and good symmetrical peaks were obtained on a Waters C18 column with the mobile phase ACN: water: Potassium Di hydrogen ortho phosphate (0.02M) 40:10:50 v/v Buffer pH 6.0 adjusted with ortho phosphoric acid.
Keywords: Pimavanserin, Inertsil ODS C18 column, RP-HPLC method