In-vitro Cytotoxic Activity of Persea Americana Miller Leaves Extracts

Narayan R Miskin*¹, Manjunatha S Katagi¹, Girish Bolakatti², Vishvakarma kamala Sunita¹ , Suchitra.M¹.
¹Bapuji Pharmacy College, SS Layout, Davangere-577004, Karnataka, India.
GM Institute of Pharmaceutical Sciences and Research, Davangere-577006, Karnataka, India.

Abstract
Phytochemistry  plays  an important role  for curing of various human diseases with slight side effects. Such medicinal plants have bioactive compounds may be primary or secondary metabolites. The present study aims to evaluate that the Invitro anticancer activity of Persea americana miller leaves extracts in different cancer cell lines. Dried Coarse powered leaves of Persea americana Miller was successively extracted with various solvents based on their polarity such as petroleum ether, chloroform, ethyl acetate and aqueous extract by maceration. Qualitative phytochemical screening was performed to detect phytochemical constituents of  the extracts. Cytotoxic activity of plant leaves extracts on MCF-7 and HeLa cells was investigated in vitro through MTT bioassay. All the extracts except EAPA has showed IC50 value of 233.7µg/ml, 182.0µg/ml, 203.7µg/ml, 147.7µg/ml, 121.0µg/ml respectively in MCF-7 cells. All the extracts have showed IC50 value 200.2µg/ml, 105.4µg/ml, 285.8µg/ml, 132.7µg/ml and 214.4µg/ml respectively in HeLa cells.
Keywords: Persea americana miller, cytotoxic activity, MTT assay.