Development and validation of HPTLC method for analysis of ursolic acid from methanol extract of mimosoups elengi L

ABOUT AUTHOR
Gayatri Ganu*¹, Tushar A. Deshmukh^2
1Shri Jagdishprasad Jhabarmal Tibrewala University, Jhunuzunu, Rajasthan
²Department of Pharmacognosy, College of Pharmacy, Faizpur

Abstract: Mimusops elengi L. (ME) is a small to large evergreen tree found all over the different parts of India. Bark of ME possesses cardio tonic, alexipharmic, stomachic, anthelmentic and astringent activity. The present investigation is an attempt to develop and validate the principal chemical constituent like ursolic acid by HPTLC. Linear ascending development with toluene: ethyl acetate: formic acid 14:7:1 (v/v) as mobile phase was performed and the UA was satisfactorily resolved with R_f value about 0.75 minute. The method is found to be precise as values were found to be 1.23 %, 1.01 % and 1.79 % respectively for system precision, method precision and intermediate precision. The proposed method is found to be accurate at 80%, 100% and 120% with average recovery of 98.25 %, 98.96% and 100.95% respectively. The proposed HPTLC method is simple, rapid, specific and accurate techniques to quantify the Ursolic acid in the presence of other constituents from the methanol extract of ME bark without compromising the accuracy.
Key words: Mimusops Elengi, Ursolic Acid, HPTLC Validation,Specificity