Development and Validation of High Performance Liquid Chromatography Method for Simultaneous Estimation of Acyclovir and Hydrocortisone in Their Combined Tablet Dosage Form

S. Samatha¹ B. Sravanthi² Dr. Gampa Vijay Kumar^3*
1KGR Institute of Technology and Management, Rampally, Kesara, Rangareddy, Telangana, India.
²Asso. Professor, KGR Institute of Technology and Management, Rampally, Kesara, Rangareddy, Telangana, India.
³Professor and Head, Dept. of Pharmacy, KGR Institute of Technology and Management, Rampally, Kesara, Rangareddy, Telangana, India.

A B S T R A C T
The proposed HPLC method was found to be simple, specific, precise, accurate, rapid and economical for simultaneous estimation of Acyclovir and Hydrocortisone in tablet dosage form. The developed method was validated in terms of accuracy, precision, linearity, robustness and ruggedness, and results will be validated statistically according to ICH guidelines. The Sample recoveries in all formulations were in good agreement with their respective label claims. From literature review and solubility analysis initial chromatographic conditions Mobile phase Phosphate buffer: Methanol PH 2.5 (60:30 v/v) were set Potassium dihydrogen orthophosphate PH 2.5 adjusted with Orthophosphoric acid, Xbridge C18  Column (250mm x 4.6mm)5µg, Flow rate 1ml min-1and temperature was ambient, eluent was scanned with PDA detector in system and it showed maximum absorbance at 258 nm. As the methanol content was increased Acyclovir and Hydrocortisone got eluted with good peak symmetric properties. The retention times for Acyclovir and Hydrocortisone was found to be 2.122 min and 3.562 min respectively. System suitability parameters were studied by injecting the standard five times and results were well under the acceptance criteria. Linearity study was carried out between50% to150 % levels, R2 value was found to be as 0.999.
Keywords: XbridgeC18, Acyclovir and Hydrocortisone, RP-HPLC