Develop and Validate a Method for the Estimation of Marbofloxacin in Pure and Pharmaceutical Dosage Form by Photo Fluorimetry

D. Chinababu*, C. Madhusudhana chetty, B. Vamsi Krishna, D. Hemanth kumar, N. Srikanth, P. Santhi, G.V. Prasanthi
Santhiram College of Pharmacy, Nandyal, Kurnool District, A.P. India.

A B S T R A C T
A simple and sensitive spectro fluorometric method was developed for the determination of Marbofloxacin in pure and its pharmaceutical dosage forms. The method is based on the solubilization of Marbofloxacin in 5:5 of ethanol& distilled water. The fluorescence produced by Marbofloxacin was measured at 486 nm after excitation at 360 nm. The different  validation parameters  like system suitability, accuracy, precision, LOQ, LOD, recovery studies, specificity, robustness, linearity, range, ruggedness were carefully studied and optimized. The method is applicable over the concentration range of 30-120 µg/ml with correlation coefficient of 0.999 for both tablet and injectable formulations. The detection limit (LOD) of Marbofloxacin was 0.303 & 0.306µg/ml for tablet and injectable dosage form and quantitation limit (LOQ) was 1.01& 1.02µg/ml for tablet and injectable dosage form respectively. The developed method is suitable for the determination of Marbofloxacin in pharmaceutical preparations with mean recoveries of 99.74-100.03 and 99.34-100.04 for Marbofloxacin tablets and injections respectively. The results were showed good precision, robustness. The method was applied for stress conditions of acid, base, peroxide, UV-light, hydrolytic conditions. This method was used for the routine analysis of marketed formulations by using photo fluorometer.
Keywords: Marbofloxacin, Photo fluorometer, Ultra-Violet