Analytical Method Development and Validation for the Simultaneous Estimation of Lesinurad and Allopurinol by RP-HPLC Method

Kodithyala Naveen Kumar¹, Sowjanya², Dr. Gampa Vijay Kumar^3
1KGR Institute of Technology and Management, Rampally, Kesara, Rangareddy, Telangana, India.
²KGR Institute of Technology and Management, Rampally, Kesara, Rangareddy, Telangana, India.
³Professor and Head, Dept. of Pharmacy, KGR Institute of Technology and Management, Rampally, Kesara, Rangareddy, Telangana, India.

ABSTRACT
On the basis of experimental results, the proposed method is suitable for the quantitative determination of Lesinurad and Allopurinol in pharmaceutical dosage form. The method provides great sensitivity, adequate linearity and repeatability. The estimation of Lesinurad and Allopurinol was done by RP-HPLC. The Phosphate buffer was pH 2.5 and the mobile phase was optimized which consists of Acetonitrile: Phosphate buffer mixed in the ratio of 80:20 % v/ v. A Symmetry C18 (4.6 x 150mm, 5mm, Make XTerra) column used as stationary phase. The detection was carried out using UV detector at 274 nm. The solutions were chromatographed at a constant flow rate of 0.8 ml/min. the linearity range of Lesinurad and Allopurinol were found to be from 25-125 mg/ml. Linear regression coefficient was not more than 0.999. The values of % RSD are less than 2% indicating accuracy and precision of the method. The percentage recovery varies from 97-102% of Lesinurad and Allopurinol LOD and LOQ was found to be within limit. The proposed method is precise, simple and accurate to determine the amount of Lesinurad and Allopurinol in formulation. High percentage of recovery shows that the method is free from the interference of excipients used in the formulation. So the method can be useful in the routine quality control of these drugs.
Keywords: Symmetry C18, Lesinurad and Allopurinol, RP-HPLC.